How is Ozone Therapy Administered?
- April 20, 2023
- Written by : Rebecca LaMarca, MSN, APN-BC, CNS
Written and medically reviewed by Rebecca LaMarca, MSN, APN-BC (PAAM, IFM, SIO) | July 13, 2026
Ozone therapy is an alternative medicine treatment that may support cellular energy production and influence antioxidant defenses, immune signaling, and oxygen delivery. Like many treatments, it can be administered in different ways, and each way comes with its own advantages and disadvantages.
No matter which administration technique you choose, it’s important to only get ozone therapy under the supervision of a medical professional. The board-certified team at IV Elements offers multiple medically supervised methods in New Jersey, New York City, and Philadelphia. Here’s what you should know about the most common ozone therapy methods and what to expect from your first session.
Important: Ozone therapy is not FDA-approved for the treatment of any condition. Always consult a licensed healthcare provider before starting any new treatment.
What are the most common ozone therapy administration techniques?
The most common ozone administration techniques include major autohemotherapy (MAH), minor autohemotherapy (MiAH), 10-pass ozone therapy, EBOO therapy, rectal or ear insufflation, and prolozone injections. Not sure which type is right for you? Read our complete guide to the types of ozone therapy first, then come back here for the step-by-step administration walkthrough.
Major autohemotherapy (MAH)
In MAH, a healthcare provider draws your blood (usually about 50–200 cc) through an IV line into a sterile bag or container. The blood is then mixed with oxygen and ozone over a few minutes. Finally, the ozonated blood is reinfused back into the vein through the same IV line. Most sessions last between 30 and 60 minutes.
Minor autohemotherapy (MiAH)
In MiAH, a healthcare provider draws a very small amount of blood, usually a few teaspoons (about 5–10 mL). As with MAH, the blood is then mixed with oxygen and ozone, but it’s mixed with them for a much shorter amount of time than in MAH. The ozonated blood is then injected into a muscle, often in the upper arm or glute. A typical session takes between 10 and 30 minutes.
10-pass ozone therapy
In 10-pass ozone therapy, a healthcare provider draws about 50–200 cc of blood into a sealed glass bottle, mixes it with oxygen and ozone under hyperbaric pressure, and reinfuses it up to ten times in a single session. Since it’s more intensive, most sessions last between 60 and 90 minutes.
Curious if 10-pass ozone therapy is right for you? Our board-certified team will design a personalized protocol based on your health and goals.
Schedule Your Consultation →EBOO therapy
In EBOO (extracorporeal blood oxygenation and ozonation) therapy, blood is continuously drawn through an IV line and circulated through a machine. Inside the machine, the blood is exposed to a mixture of oxygen and ozone and then returned to the body through a second IV line. Like the 10-pass method, it often takes between 60 and 90 minutes.
Rectal or ear insufflation
In rectal insufflation, ozone gas is introduced into the rectum via a catheter and then absorbed through the intestinal lining. In ear insufflation, a very small amount of ozone gas is passed through a humidifier and delivered into the ear canal through a small device placed over or near the ear. Both methods tend to take about 10 to 30 minutes.
Prolozone injections
For a prolozone injection, a healthcare provider injects an oxygen-ozone mixture into injured joints, tendons, or ligaments to promote healing. The mixture is usually injected alongside a local anesthetic as well as vitamins and minerals. It’s typically complete within 10 to 30 minutes.
What happens during an ozone session?
For a full breakdown of what happens before, during, and after an ozone therapy session, see our complete guide to what to expect at your ozone therapy session.
How long does a session take?
Here are the average session lengths for each administration method:
| Method | Typical Session Length |
|---|---|
| Ear insufflation | 10–20 minutes |
| Rectal insufflation | 10–30 minutes |
| MiAH (minor autohemotherapy) | 10–30 minutes |
| Prolozone injections | 10–30 minutes |
| MAH (major autohemotherapy) | 30–60 minutes |
| 10-pass ozone therapy | 60–90 minutes |
| EBOO | 60–90 minutes |
The exact amount of time it takes depends on factors like your health, treatment goals, and whether it’s your first session or a repeat session.
Not sure which ozone therapy method is right for you?
Book a ConsultationIs ozone therapy painful?
Ozone therapy usually isn’t described as painful, but you might feel some discomfort depending on the method. In ozone therapy IV procedures like MAH, 10-pass, or EBOO, you might feel a mild pinch or sting when the IV is inserted. You may also feel some mild pressure at the IV site during treatment along with mild fatigue once it’s over.
If you get MiAH or prolozone injections, you might experience some muscle soreness at the injection site. Finally, rectal insufflation may involve some mild cramping or bloating, while ear insufflation could cause a little pressure or irritation in the ear canal.
At IV Elements, our nurses supervise all ozone therapy sessions to ensure your comfort and reduce your risk of unpleasant side effects.
Schedule your ozone consultation with IV Elements
Now that you know what to expect during your ozone session, you can book a consultation with IV Elements to create your personalized treatment plan! Led by Rebecca LaMarca (MSN, APN-BC), we offer multiple medically supervised ozone treatments that make it easy to meet your health goals, including MAH, 10-pass ozone therapy, EBOO, and prolozone injections.
Start your ozone journey at one of our peaceful drip lounges in Hoboken (serving Jersey City and Manhattan), Marlton (Cherry Hill and Philadelphia suburbs), Parsippany (Morris and Essex Counties), or Asbury Park (Monmouth County and the Jersey Shore), plus our partnership location at Namaste Spa in Ridgewood. We also have a convenient mobile service. As proud members of the Physicians’ Association for Anthroposophic Medicine (PAAM), the Society for Integrative Oncology (SIO), and the Institute for Functional Medicine (IFM), we’re committed to providing safe, evidence-based care. Schedule your consultation today!
Frequently Asked Questions
How does ozone therapy work?
Ozone therapy works by exposing blood or body tissues to a mixture of oxygen and ozone. The mixture creates temporary oxidative signals that may influence antioxidant pathways, immune responses, and oxygen utilization.
How do you mix ozone for IV?
In IV ozone therapy like MAH and EBOO, practitioners generally make medical-grade ozone using a calibrated ozone generator that turns oxygen into an oxygen-ozone mixture via electrical discharge.
What’s the difference between IV and rectal ozone?
IV ozone therapy and rectal ozone therapy differ in how the ozone enters your body. In IV ozone therapy, your blood is drawn through an IV line, exposed to a mixture of oxygen and ozone outside the body, and then reinfused into your bloodstream. In rectal ozone therapy, an oxygen-ozone gas mixture is delivered into the rectum via a catheter so it can be absorbed through your intestinal lining. It’s usually considered a more mild and gradual way to deliver ozone compared to IV therapy.
Where can I get ozone therapy in NJ?
You can get ozone therapy in NJ at IV Elements’ drip lounges in Hoboken, Parsippany, Asbury Park, and Marlton, plus our partnership location at Namaste Spa in Ridgewood.
Can I get mobile ozone therapy in NJ?
Some types of ozone therapy are available through IV Elements’ mobile service depending on availability and your treatment needs. We offer mobile treatments throughout New Jersey, New York City, Philadelphia, and Bucks County, PA.
References
- Ozone IV Therapy: Advanced Healing with Ozone Therapy. (2022, June 13). IV Elements. https://ivelements.net/ozone-therapy
- Lietz-Kijak, D., Skibicka, K., Skomro, P., Brodkiewicz, A., Budkowska, M., Kijak, K., Gronwald, H., Kijak, K., Szczegielniak, J., Bogacz, K., Bitenc-Jasiejko, A., Szczucka, L., Garstka, A. A., & Wójcik, M. (2026). Medical ozone: mechanisms, biological effects and clinical evidence—a narrative review. Frontiers in Medicine, 13. https://doi.org/10.3389/fmed.2026.1859221
- Ogut, E. (2025). Mechanisms and therapeutic potential of ozone therapy in enhancing vasculogenesis and angiogenesis through growth factor activation and oxidative signaling pathways. Discover Medicine, 2(1). https://doi.org/10.1007/s44337-025-00491-7
- Did You Know Ozone Can Be Administered in Several Ways? (2023, May 11). IV Elements. https://ivelements.net/blog/types-of-ozone-therapy
- 10 Pass IV Ozone. (2025, November 6). IV Elements. https://ivelements.net/10-pass-ozone-therapy
- Benefits of EBOO Ozone. (2024, November 20). IV Elements. https://ivelements.net/blog/eboo-ozone
- 10-Pass Ozone Therapy Benefits. (2023, April 20). IV Elements. https://ivelements.net/blog/benefits-of-10-pass-ozone-therapy
- Schedule Your IV Therapy At-Home | Book IV Drip Now. (2024, November 4). IV Elements. https://ivelements.net/book-now-1
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Ozone IV Therapy Treatments
Ozone is a type of oxygen. Instead of two oxygen atoms connected to one another, ozone consists of three atoms. According to decades of research, this third atom brings a lot to the table.
